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. 2024 Nov 19;15(11):844. doi: 10.1038/s41419-024-07245-w

Fig. 6. The detrimental impact of hsa-miR-659-3p on RON-mediated migration and invasion of bladder cancer cells.

Fig. 6

A The levels of miR-659-3p were assessed in normal bladder tissues (n = 7) and cancer samples (n = 15) using quantitative real-time polymerase chain reaction (qRT-PCR). B The expression of miR-659-3p was evaluated in various bladder cancer cell lines, with the 5637 cell line serving as the control. C Following transfection with miR-659-3p mimics or negative control in 5637 cells, and with miR-659-3p inhibitor or negative control in J82 cells, the expression of RON was confirmed through western blot analysis. D The migration ability was tested by wound-healing assay. E The invasion ability was evaluated by trans-well assay. (Scale bar 1 µm). F J82 cells were transduced with miR-659-3p mimics and a RON overexpression plasmid, followed by validation of RON expression using Western blot analysis. G The migration ability was tested by wound healing assay. H The invasion ability was evaluated by trans-well assay. (Scale bar 1 µm) Experiments in B–H were repeated three times (n = 3).