Figure 1.

Phylogenetic tree of Leptospira DNA extracted from raccoons and characterized based on the lfb1 gene sequences (bootstrap analysis involving 1 000 replicates and 34 nucleotide sequences). The evolutionary distances (unit = base substitutions per site) were computed using the maximum composite likelihood method [10] and MEGA11 software. The pink circles represent the amplicons from the samples. The contigs are identified using a coding system with the format ‘RL NN M’: where NN indicates sample geographical origin (northeastern France = A and U, southwestern France = G) plus ID number and M indicates sample tissue type (urine = U, kidney = R).