KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Arginase-1 | Cell Signaling Technology | Cat# 93668; RRID:AB_2800207 |
| CD80 | ProSci | Cat#8679 |
| Histone H3 | Abcam | Cat# ab5103; RRID:AB_304752 |
| MPO | R and D Systems | Cat# AF3667; RRID:AB_2250866 |
| MRC1 | Cell Signaling Technology | Cat# 24595; RRID:AB_2892682 |
| F4/80 | Cell Signaling Technology | Cat# 70076; RRID:AB_2799771 |
| COX2 | Abcam | Cat# ab15191; RRID:AB_2085144 |
| iNOS | Cell Signaling Technology | Cat# 2982; RRID:AB_1078202 |
| GCSFR | Bioss | Cat# bs-2574R; RRID:AB_10857313 |
| CD163 | Cell Signaling Technology | Cat# 68922; RRID:AB_3105898 |
| Ly6G | BD Pharmingen | Cat# 551459; RRID:AB_394206 |
| IgG (H + L) Alexa Fluor™ 488 | Thermo-Fisher | Cat# A-11008 (also A11008); RRID:AB_143165 |
| IgG (H + L) Alexa Fluor™ 647 | Thermo-Fisher | Cat# A-21247; RRID:AB_141778 |
| IgG (H + L) Alexa Fluor™ 555 | Thermo-Fisher | Cat# A-31572 (also A31572); RRID:AB_162543 |
| CD16/32 | BioLegend | Cat# 101320 (also 101319); RRID:AB_1574975 |
| CD45 | BioLegend | Cat# 103130 (also 103129); RRID:AB_893339 |
| F4/80 | BioLegend | Cat# 123149; RRID:AB_2564589 |
| CD11b | BioLegend | Cat# 101220 (also 101218); RRID:AB_493546 |
| CD31 | Cell Signaling Technology | Cat#77699; RRID:AB_2722705 |
| G-CSF | R and D Systems | Cat# MAB414; RRID:AB_2085954 |
| Ki-67 | Cell Signaling | Cat# 9129; RRID: AB_2687446 |
| Elastase | Abcam | Cat# ab68672; RRID:AB_1658868 |
| Cytokeratin 14 | Abcam | Cat# ab7800; RRID:AB_306091 |
| keratin 6 | Sigma | Cat# SAB5500131 |
| Phospho-Histone H3 | Cell Signaling Technology | Cat# 53348; RRID: AB_2799431 |
| CD45 | BioLegend | Cat# 103130; RRID:AB_893339 |
| F4/80 | BioLegend | Cat# 123149; RRID: AB_2564589 |
| CD11b | BioLegend | Cat# 101218; RRID: AB_389327 |
| GR-1 | BioLegend | Cat# 127617; RRID: AB_1877262 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Gibco RPMI media | Thermo Fisher Scientific | 12633012 |
| Dnase I Recombinant, Rnase-free | Millipore Sigma | 4716728001 |
| 1x HBSS | Corning | 21–023-CV |
| HEPES-Buffer 1M, pH 7.5 | Boston BioProducts | BBH-75-K-500 |
| Liberase TL Research Grade | Thermo Fisher Scientific | NC1328423 |
| FBS | Corning | 35–010-CV |
| Bovine Serum Albumin | Sigma-Aldrich | A7906–50G |
| 70 μm Strainer | Fisher Scientific | 22–363-548 |
| 40 μm Strainer | Corning | 431750 |
| 1x DPBS | Gibco | 14190–136 |
| TRIzol | Thermo Fisher Scientific | 15596026 |
| UltraPure Phenol:Chloroform:Isoamyl Alcohol | Thermo Fisher Scientific | 15593031 |
| 0.5 M EDTA, pH 8.0 | Invitrogen | 15575–038 |
| Paraformaldehyde Solution, 4% in PBS | Thermo Scientific | J19943-K2 |
| DMSO | Fisher Scientific | 200–664-3 |
| OCT | Fisher Health Care | 4585 |
| Agencourt RNA Clean XP Beads | Beckman Coulter | A63987 |
| SPRI Beads | Beckman Coulter | B23318 |
| G-CSF | Med Chem Express | HY-P70608 |
| Magnetic Conjugation kit | Abcam | ab269890 |
| Mouse G-CSF Quantikine ELISA kit | R and D Systems | MCS00 |
| DeadEnd Flurometric TUNERL System | Promega | G3250 |
| Picrosirius red | Sigma-Aldrich | 365548 |
| Zombie GreenTM cell viability dye | Biolegend | 423111 |
| TruStain FcX anti-mouse CD16/32 | Biolegend | 101320, clone 93 |
|
| ||
| Critical commercial assays | ||
|
| ||
| Chromium Next GEM Single Cell 3′ Kit v3.1 | 10x Genomics | 1000268 |
| Chromium Next GEM Chip G Single Cell Kit | 10x Genomics | 1000120 |
| Dual Index Kit TT Set A | 10x Genomics | 1000215 |
| Chromium i7 Sample Index Plate | 10x Genomics | 120262 |
| NEBNext Ultra DNA Library Prep Kit for Illumina | NEB | E7370 |
| Nextseq 500/550 Mid-Output v2.5 Kit | Illumina | 200024904 |
| NEBNext Low Input Prep Kit | NEB | E7630L |
|
| ||
| Deposited data | ||
|
| ||
| Raw scRNA-seq data | This Study | GEO: GSE245864 |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| Mouse: Wild-type C57BL/6J | The Jackson Laboratory (JAX) | #000664 |
| Mouse: CXCR2−/− | The Jackson Laboratory (JAX) | #2724 |
| Mouse: CXCR2f/f | The Jackson Laboratory (JAX) | #24638 |
| Mouse: K14-Cre | The Jackson Laboratory (JAX) | #4782 |
| Mouse: COL1A1-CreER | The Jackson Laboratory (JAX) | #27751 |
| Mouse: LysM-Cre | The Jackson Laboratory (JAX) | #4781 |
| Mouse: IL-17A−/− | The Jackson Laboratory (JAX) | #35717 |
| Mouse: Pad4−/− | The Jackson Laboratory (JAX) | #030315 |
|
| ||
| Software and algorithms | ||
|
| ||
| cellranger pipeline 5.0.1 | 10x Genomics | https://support.10xgenomics.com/single-cell/software/overview/welcome |
| Seurat 4.3.0 | Hao et al., 202139 | https://github.com/satijalab/seurat/ |
| FACSDiva 7 | BD Biosciences | https://www.bdbiosciences.com/en-us |
| FlowJo 10.4.2 | VD Biosciences | NA |
| Prism8 | GraphPad Inc. | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ | NIH, USA | https://imagej.nih.gov/ij/ |
| DESeq2 v | Bioconductor project, USA | https://bioconductor.org/packages/DESeq2/ |
| ggplot2 v3.2.1 | Wickham40 | https://CRAN.R-project.org/package=ggplot2 |
| ComplexHeatmap | Bioconductor project, USA | https://bioconductor.org/packages/ComplexHeatmap/ |
| fgsea | Bioconductor project, USA | https://bioconductor.org/packages/fgsea/ |
| R | The R Foundation | https://www.r-project.org |
| UMAP | https://github.com/lmcinnes/umap | |
| Adobe Illustrator 2022 | Adobe | N/A |
| CellChat | Jin et al., 202141 | http://www.cellchat.org/ |
| DoubletFinder | McGinnis et al.42 | https://github.com/chris-mcginnis-ucsf/DoubletFinder |
| clustree | CRAN | https://cran.r-project.org/web/packages/clustree/index.html |
| Enrichplot | Bioconductor project, USA | https://bioconductor.org/packages/release/bioc/html/enrichplot.html |