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. 2024 Oct 11;21:36. doi: 10.25259/Cytojournal_31_2024

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© 2024 The Author(s). Published by Scientific Scholar

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Figure 3: — Effects of 5-Aza-2’-deoxycytidine demethylation on expressions of PTEN and methylation related factors, and PI3K/AKT signaling pathway related proteins. (a) PTEN mRNA expression by RT-PCR; (b) DNMT1, DNMT3a, MBD2, and MeCP2 mRNA expressions by RT-PCR; (c) PTEN, PI3K, AKT, and mTOR protein expressions by western blot; (d) statistical analysis of protein expressions. (n = 3). (*P < 0.05, **P < 0.001). (PTEN: Phosphatase and tensin homolog deleted on chromosome ten; RT-PCR: reverse transcription-polymerase chain reaction.)