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. 2024 Oct 23;27(11):111240. doi: 10.1016/j.isci.2024.111240
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

FITC anti-human Lineage Cocktail Biolegend Cat#348801
APC anti-human CD294 (CRTH2) Antibody Biolegend Cat#350110
APC/Cyanine7 anti-human CD45 Antibody Biolegend Cat#368516
PE anti-human CD127 Biolegend Cat#351340
Biotin anti-mouse CD3ε (clone 145-2C11) Biolegend Cat#100304
Biotin anti-mouse CD4 (clone GK1.5) Biolegend Cat#100404
Biotin anti-mouse CD8α (clone 53-6.7) Biolegend Cat#100704
Biotin anti-mouse CD11c (clone N418) Biolegend Cat#117304
Biotin anti-mouse FceRIα (clone MAR-1) Biolegend Cat#134304
Biotin anti-mouse NK1.1 (clone PK136) Biolegend Cat#108704
Biotin anti-mouse CD19 (clone 6D5) Biolegend Cat#115504
Biotin anti-mouse TER119 (clone TER-119) BioLegend Cat#116204
Biotin anti-mouse CD5 (clone 53-7.3) BioLegend Cat#100604
Biotin anti-mouse F4/80 (clone BM8.1) Tonbo Biosciences Cat#30-4801-U500
Biotin anti-mouse Gr1 (clone RB6-8C5) Tonbo Biosciences Cat#30-5931-U500
APC-conjugated streptavidin BioLegend Cat#405207
PE-conjugated T1/ST2 (clone DIH9) BioLegend Cat#145304
Anti-Mouse Siglec-F PE (clone E50-2440) BD Biosciences Cat#552126
Anti-Mouse CD11c PE-Cy7 (clone N418) Tonbo Biosciences Cat#60-0114-U100
Anti-Mouse CD45 APC-Cy7 (clone 30-F11) BD Biosciences Cat#561037
PE anti-human Ki-67 Antibody Biolegend Cat#350504
PE anti-mouse Ki-67 Antibody Biolegend Cat#652404

Chemicals, peptides, and recombinant proteins

PamsCSK4 InvivoGen Cat#tlrl-pms
Poly(I:C) InvivoGen Cat#tlrl-pic
Flagellin InvivoGen Cat#tlrl-bsfla
R848 InvivoGen Cat#tlrl-r848-1
CpG-A (ODN 2336, human)CpG-A (ODN 1585, mouse) InvivoGen Cat#tlrl-2336-1 Cat#tlrl-1585
ISR23 IDT N/A
LPS from E. coli 0127:B8 LPS from E. coli 055:B5 LPS from P. aeruginosa 10 LPS from S. typhimurium Sigma Cat#L4516
Cat#L6529
Cat#L8643
Cat#L6143
CRX-527 InvivoGen Cat#tlrl-crx-527
LPS-RS Ultrapure InvivoGen Cat#tlrl-prslps
Human IL1RL1/ST2 protein Sino Biologicl Cat#13034-H08H
Bay 11-7082 EMD Millipore Cat#196870
JAK inhibitor 1 EMD Millipore Cat#420099
PMA and Brefeldin A Sigma Cat#P1585
Cat#B6542
SPHERO AccuCount Fluorescent Spherotech Cat#ACFP-70-5
Fixable Viability Dye eFluor 506 Invitrogen Cat#65-0866-14
Recombinant Human IL-2 PeproTech Cat#200-02
Recombinant Human IL-7 PeproTech Cat#200-07
Recombinant Human IL-33 PeproTech Cat#200-33
Recombinant Murine IL-2 PeproTech Cat#212-12
Recombinant Murine IL-7 PeproTech Cat#217-17
Recombinant Murine IL-33 PeproTech Cat#210-33
CFSE Cell Division Tracker Kit Biolegend Cat#423801
1-Step™ TMB ELISA Substrate Solutions Thermo Scientific Cat#34028
Cyto-Fast™ Fix/Perm Buffer Set Biolegend Cat#426803
True-Nuclear™ Transcription Factor Buffer Set Biolegend Cat#4244011
CFSE Cell Division Tracker Kit Biolegend Cat#423801
Human IL-5 Uncoated ELISA Kit Thermo Scientific Cat#88-7056-88
Human IL-13 Uncoated ELISA Kit Thermo Scientific Cat#88-7439-88
Mouse IL-5 Uncoated ELISA Kit Thermo Scientific Cat#88-7054-88
Mouse IL-13 Uncoated ELISA Kit Thermo Scientific Cat#88-7137-88

Deposited data

RNA-seq raw data generated for this paper Sequence Read Archive (SRA) of NCBI (https://trace.ncbi.nlm.nih.gov/Traces/?view=study&acc=SRP534046) SRR30760103
SRR30760104
SRR30760105
SRR30760106

Experimental models: Organisms/strains

Rag1−/− Jackson Laboratory Cat#002216
Rag2−/−γc−/− Jackson Laboratory Cat#014593
NSG Jackson Laboratory Cat#005557
Il1rl1−/− GemPharmatech Cat#T005974

Oligonucleotides

GAPDH ATGACATCAAGAAGGTGGTG; CATACCAGGAAATGAGCTTG N/A
IL-4 ACTTTGAACAGCCTCACAGAG; TTGGAGGCAGCAAAGATGTC N/A
IL-5 AGCTGCCTACGTGTATGCCA; CAGGAACAGGAATCCTCAGA N/A
IL-13 TGAGGAGCTGGTCAACATCA; CAGGTTGATGCTCCATACCAT N/A
TNF-α CCTGGTATGAGCCCATCTATCTG; TAGTCGGGCCGATTGATCTC N/A

Software and algorithms

Graphpad Prism 8 Prism N/A
FlowJo Version 9 BD Biosciences N/A