REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
FITC anti-human Lineage Cocktail | Biolegend | Cat#348801 |
APC anti-human CD294 (CRTH2) Antibody | Biolegend | Cat#350110 |
APC/Cyanine7 anti-human CD45 Antibody | Biolegend | Cat#368516 |
PE anti-human CD127 | Biolegend | Cat#351340 |
Biotin anti-mouse CD3ε (clone 145-2C11) | Biolegend | Cat#100304 |
Biotin anti-mouse CD4 (clone GK1.5) | Biolegend | Cat#100404 |
Biotin anti-mouse CD8α (clone 53-6.7) | Biolegend | Cat#100704 |
Biotin anti-mouse CD11c (clone N418) | Biolegend | Cat#117304 |
Biotin anti-mouse FceRIα (clone MAR-1) | Biolegend | Cat#134304 |
Biotin anti-mouse NK1.1 (clone PK136) | Biolegend | Cat#108704 |
Biotin anti-mouse CD19 (clone 6D5) | Biolegend | Cat#115504 |
Biotin anti-mouse TER119 (clone TER-119) | BioLegend | Cat#116204 |
Biotin anti-mouse CD5 (clone 53-7.3) | BioLegend | Cat#100604 |
Biotin anti-mouse F4/80 (clone BM8.1) | Tonbo Biosciences | Cat#30-4801-U500 |
Biotin anti-mouse Gr1 (clone RB6-8C5) | Tonbo Biosciences | Cat#30-5931-U500 |
APC-conjugated streptavidin | BioLegend | Cat#405207 |
PE-conjugated T1/ST2 (clone DIH9) | BioLegend | Cat#145304 |
Anti-Mouse Siglec-F PE (clone E50-2440) | BD Biosciences | Cat#552126 |
Anti-Mouse CD11c PE-Cy7 (clone N418) | Tonbo Biosciences | Cat#60-0114-U100 |
Anti-Mouse CD45 APC-Cy7 (clone 30-F11) | BD Biosciences | Cat#561037 |
PE anti-human Ki-67 Antibody | Biolegend | Cat#350504 |
PE anti-mouse Ki-67 Antibody | Biolegend | Cat#652404 |
Chemicals, peptides, and recombinant proteins | ||
PamsCSK4 | InvivoGen | Cat#tlrl-pms |
Poly(I:C) | InvivoGen | Cat#tlrl-pic |
Flagellin | InvivoGen | Cat#tlrl-bsfla |
R848 | InvivoGen | Cat#tlrl-r848-1 |
CpG-A (ODN 2336, human)CpG-A (ODN 1585, mouse) | InvivoGen | Cat#tlrl-2336-1 Cat#tlrl-1585 |
ISR23 | IDT | N/A |
LPS from E. coli 0127:B8 LPS from E. coli 055:B5 LPS from P. aeruginosa 10 LPS from S. typhimurium | Sigma | Cat#L4516 Cat#L6529 Cat#L8643 Cat#L6143 |
CRX-527 | InvivoGen | Cat#tlrl-crx-527 |
LPS-RS Ultrapure | InvivoGen | Cat#tlrl-prslps |
Human IL1RL1/ST2 protein | Sino Biologicl | Cat#13034-H08H |
Bay 11-7082 | EMD Millipore | Cat#196870 |
JAK inhibitor 1 | EMD Millipore | Cat#420099 |
PMA and Brefeldin A | Sigma | Cat#P1585 Cat#B6542 |
SPHERO AccuCount Fluorescent | Spherotech | Cat#ACFP-70-5 |
Fixable Viability Dye eFluor 506 | Invitrogen | Cat#65-0866-14 |
Recombinant Human IL-2 | PeproTech | Cat#200-02 |
Recombinant Human IL-7 | PeproTech | Cat#200-07 |
Recombinant Human IL-33 | PeproTech | Cat#200-33 |
Recombinant Murine IL-2 | PeproTech | Cat#212-12 |
Recombinant Murine IL-7 | PeproTech | Cat#217-17 |
Recombinant Murine IL-33 | PeproTech | Cat#210-33 |
CFSE Cell Division Tracker Kit | Biolegend | Cat#423801 |
1-Step™ TMB ELISA Substrate Solutions | Thermo Scientific | Cat#34028 |
Cyto-Fast™ Fix/Perm Buffer Set | Biolegend | Cat#426803 |
True-Nuclear™ Transcription Factor Buffer Set | Biolegend | Cat#4244011 |
CFSE Cell Division Tracker Kit | Biolegend | Cat#423801 |
Human IL-5 Uncoated ELISA Kit | Thermo Scientific | Cat#88-7056-88 |
Human IL-13 Uncoated ELISA Kit | Thermo Scientific | Cat#88-7439-88 |
Mouse IL-5 Uncoated ELISA Kit | Thermo Scientific | Cat#88-7054-88 |
Mouse IL-13 Uncoated ELISA Kit | Thermo Scientific | Cat#88-7137-88 |
Deposited data | ||
RNA-seq raw data generated for this paper | Sequence Read Archive (SRA) of NCBI (https://trace.ncbi.nlm.nih.gov/Traces/?view=study&acc=SRP534046) | SRR30760103 SRR30760104 SRR30760105 SRR30760106 |
Experimental models: Organisms/strains | ||
Rag1−/− | Jackson Laboratory | Cat#002216 |
Rag2−/−γc−/− | Jackson Laboratory | Cat#014593 |
NSG | Jackson Laboratory | Cat#005557 |
Il1rl1−/− | GemPharmatech | Cat#T005974 |
Oligonucleotides | ||
GAPDH | ATGACATCAAGAAGGTGGTG; CATACCAGGAAATGAGCTTG | N/A |
IL-4 | ACTTTGAACAGCCTCACAGAG; TTGGAGGCAGCAAAGATGTC | N/A |
IL-5 | AGCTGCCTACGTGTATGCCA; CAGGAACAGGAATCCTCAGA | N/A |
IL-13 | TGAGGAGCTGGTCAACATCA; CAGGTTGATGCTCCATACCAT | N/A |
TNF-α | CCTGGTATGAGCCCATCTATCTG; TAGTCGGGCCGATTGATCTC | N/A |
Software and algorithms | ||
Graphpad Prism 8 | Prism | N/A |
FlowJo Version 9 | BD Biosciences | N/A |