Figure 5.

Assessing the stereoselectivity of VcGluP. (A and B) Derivatives of the unfolding curves (dF/dT) for VC0430 in the absence of ligand and the presence of (A) 1 mM L- and D-glutamate, and (B) 1, 5, and 15 mM L- and D-glutamine. (C) Thermostabilization of VcGluP in the presence of L- and D-glutamate and glutamine. Error bars represent standard deviation and individual data points are shown as red circles. (D) Representative binding curve for D-glutamate binding to VcGluP. Information regarding replicates of the unfolding curve in A and B can be found in Table S2.