Fig. 6.

RNA-sequencing of AD-MSCs from sub-groups a and b. A Principal Component Analysis (PCA) plot showing the distribution of samples from sub-group a and b along the first two principal components (PC1 and PC2). The percentages on the axes represent the proportion of total variance explained by PC1 and PC2. B Volcano Plot of 10 individuals’ AD-MSCs from sub-groups a and b. |Log2(Fold change)| ≥ 1, FDR < 0.05, 238 genes (green plots) were down-regulated and 66 genes (red plots) were up-regulated ( sub-group a as control). C Clustering heat map of 10 individuals’ AD-MSCs from sub-groups a and b (red arrows showed CCNB1, CCNB2, CDK1, and CDC20). D–G GO and KEGG enrichment analysis of different expression genes. H Gene Set Enrichment Analysis (GSEA) results illustrating the cell cycle pathway gene sets in sub-group a and b. ES (enrichment score), p-value and FDR were shown