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. 2024 Nov 19;21:303. doi: 10.1186/s12974-024-03287-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — Representative images of IL-1R1 expression on glutamatergic neurons in different brain sections. (A) Representative images of tdTomato immunolabeling in Vglut2-Cre-Il1r1^r/r mice, highlighting the cortex and olfactory areas. MOp, primary motor area; ORBvl, orbital area, ventrolateral part; Alv, agranular insular area, ventral part; PIR, piriform area; AON, anterior olfactory nucleus. The dashed squares indicate areas with high neuronal IL-1R1 expression and are magnified. Scale bar = 200 μm (B) Representative images of tdTomato immunolabeling in the Vglut2-Cre-Il1r1^r/r somatosensory areas, visceral areas, and piriform area. SSp, primary somatosensory area; SSs, supplementary somatosensory area; VISC, visceral areas; PIR, piriform area. Scale bar: 200 μm (C) Representative images of DAB labeling in various thalamic nuclei of the Vglut2-Cre-Il1r1^r/r mouse. AD, anterodorsal nucleus; MD, mediodorsal nucleus of thalamus; PVT, paraventricular nucleus of thalamus; AM, anteromedial nucleus. Scale bar: 200 μm (D) Representative images of tdTomato immunolabeling in Vglut2-Cre-Il1r1^r/r mouse dentate gyrus (DG), central lateral nucleus of the thalamus (CL), and basomedial amygdalar nucleus (BMA). Scale bar = 200 μm