TABLE 1.
Frequency of de novo mutations in single clones of P. putida in monoculture and co-culture after 200 generationsa
| Clone | Morph. | Position | Mutation | Gene | Freq. in pop. |
Description |
|---|---|---|---|---|---|---|
| Mono. 1 | Large | 3,987,060 | Δ1,094 bp | oplB/oplA | <5% | –c |
| Mono. 2 | Large | 4,964,741 | 2 bp→ Cb | fleQ | 100% | Transcriptional regulator FleQ |
| 5,715,528 | N65N | tatB | 100% | TatB | ||
| 6,029,059 | A134A | kauB | 100% | KauB | ||
| Mono. 3 | Large | –c | – | – | – | – |
| Mono. 4 | Large | 1,843,830 | D322H | gacS | 100% | Sensor protein GacS |
| 4,978,570:1 | +C | flgH | 100% | Flagellar L-ring protein | ||
| Co-culture 1 | Large | 4,964,681 | A226V | fleQ | 23.1% | Transcriptional regulator FleQ |
| Co-culture 1 | Small | – | – | – | – | – |
| Co-culture 2 | Large | 4,964,300 | L353Q | fleQ | 35.6% | Transcriptional regulator FleQ |
| Co-culture 2 | Small | 3,990,098 | V128G | PP_RS18280 | <5% | Helix-turn-helix domain-containing protein |
| Co-culture 3 | Large | 4,635,453 | Y101* | uvrY | <5% | UvrY/SirA/GacA family response regulator transcription factor |
| Co-culture 3 | Small | 3,987,689 | Δ1 bp | olpA | 12.9% | Hydantoinase A/oxoprolinase family protein |
| Co-culture 4 | Large | – | – | – | – | – |
| Co-culture 4 | Small | 4,025,185 | A172T | PP_RS18445 | <5% | HlyD family efflux transporter periplasmic adaptor subunit |
a
Monoculture data are extracted from our previous study (28); co-culture data were generated in this study.
b
Deletion of first base and substitution of second base with C.
c
– indicates that no mutations were found.