Fig. 4. Evaluation of CO-like activities of ATT-Cu and TT-Cu. (A) UV-vis spectra of 3,5-DTBC, 3,5-DTBQ and nanozyme-catalyzed oxidation of 3,5-DTBC in air-saturated PBS (pH = 8)/CH₃CN solution; (B) specific activities; (C) Michaelis–Menten curves; (D) kinetic parameters (K_m, K_cat and K_cat/K_m); (E) recyclability; (F) comparison of the kinetic parameters (K_m, K_cat and K_cat/K_m) of ATT-Cu with reported CO-like artificial enzymes.