Fig. 4. Solid-phase tag based scRNA-seq methods. (A) Schematic workflow of Drop-seq for highly parallel single-cell transcriptome sequencing based on droplet microfluidics. This figure has been reproduced from Cell, 2015, 161, 1202–1214, with permission from Elsevier, copyright 2015.⁷ (B) Schematic workflow of Well-Paired-seq that utilizes thousands of microwells for single-cell RNA sequencing. This figure has been reproduced from Small Methods, 2022, 6, e2200341, with permission from Wiley-VCH GmbH, copyright 2022.¹² (C) Schematic workflow of Paired-seq for highly parallel scRNA-seq, which utilizes hydrodynamic traps for single-cell isolation and valve/pump structure for liquid control. This figure has been reproduced from Genome Biol, 2016, 17, 77, with permission from Springer Nature Publishing, copyright 2020.⁵³ (D) Disco integrates a valve-based strategy with droplet microfluidics for pairing of a single cell and a bead. This figure has been reproduced from Nat Methods, 2022, 19, 323–330, with permission from Springer Nature Publishing, copyright 2022.¹⁷.