Fig. 9. (A) Schematic workflow of scEU-seq which integrates 5-EU labeling, click chemistry-based biotinylation, and MARS-seq to simultaneously quantify nascent and pre-existing transcripts in thousands of single cells. This figure has been reproduced from Science, 2020, 367, 1151–1156, with permission from American Association for the Advancement of Science, copyright 2020.8 (B) Schematic workflow of TEMPOmap that employs 5-EU metabolic labeling and a tri-probe set to spatially and temporally resolve scRNA-seq. This figure has been reproduced from Nat Methods, 2023, 20, 695–705, with permission from Springer Nature Publishing, copyright 2023.13.
