Table 2.

Drug-resistance cassettes used for λ Red recombineering

Antibiotic	Gene(s)	Primer pair	Drug conc. (μg/ml)	Cassette length (base pairs)
Ampicillin	Tn3 bla	5′-CGCGGAACCCCTATTTGTTT-3′ 5′-GGTCTGACAGTTACCAATGC-3′	50	975
Tetracycline	Tn10 tetRA	5′-CTCGACATCTTGGTTACCGT-3′ 5′-CGCGGAATAACATCATTTGG-3′	7	1,996
Kanamycin	Tn903 aph (type I)	5′-CACGTTGTGTCTCAAAATCTC-3′ 5′-TACAACCAATTAACCAATTCTG-3′	20	944
Kanamycin	Tn5 aph (type II)	5′-TATGGACAGCAAGCGAACCG-3′ 5′-TCAGAAGAACTCGTCAAGAAG-3′	20	949
Chloramphenicol	Tn9 cat	5′-TGAGACGTTGATCGGCACGT-3′ 5′-ATTCAGGCGTAGCACCAGGC-3′	10	822
Spectinomycin	Tn21 aadA	5′-AAACGGATGAAGGCACGAA-3′ 5′-TTATTTGCCGACTACCTTGG-3′	20	1,080
Gentamicin	Tn1696 aacC	5′-CGAATCCATGTGGGAGTTTA-3′ 5′-TTAGGTGGCGGTACTTGGGT-3′	10	616