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Enteroid cell activity and intact enteroid analysis from d1 to d7 in culture. (A) Cell activity measured by PrestoBlue analysis. (B) Number of intact enteroids. Measurements of all other days were normalized to d1 (100%) as the relative viability or relative count. Data are expressed as the mean ± SEM from at least six replicate wells. Different letters indicate p ≤ 0.05 among culture days.
