FIGURE 5.

Evaluation of glucose transport in enteroids at d2, d4, and d6 in culture. (A, B) 2NBDG retention in the enteroids pretreated with DMSO control at ×100 and ×400 magnification, respectively. (C, D) 2NBDG retention in the enteroids pretreated with SGLT1 glucose transporter blocker phlorizin 0.5 mM at ×100 and ×400 magnification, respectively.(E) Data are presented as the mean ± SEM from at least six replicate wells. Two-way ANOVA showed culture days effect P ≦ 0.05, blocked effect P ≦ 0.05, interaction p = 0.06. # indicates P ≦ 0.10. * indicates P ≦ 0.05, and *** indicates P ≦ 0.0001 compared between PZ and Con groups, or among culture days in Con groups.