Fig. 3.

Analysis of the 2D structures of developed aptamers. (A) Prediction of secondary structure was carried out using the software available at: https://rna.urmc.rochester.edu/RNAstructureWeb/index.html (prediction of secondary structure common to more sequences) and confirmed by the software available at: http://www.unafold.org. (B) Putative loops (grey). Overhang found in P2c2s whose removal does not affect the affinity towards PD1 is highlighted by a black frame. Regions from positional scanning are marked as numbers corresponding to Fig. 2.