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. 2024 Oct 25;143(12):1445–1457. doi: 10.1007/s00439-024-02708-8

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

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Fig. 4 — DDX41 Regulates Periostin expression. (A) Quantitative real-time PCR analysis of Periostin (POSTN) gene expression using two different Taqman Probes shows significantly increased expression of POSTN in the proband-derived fibroblasts compared to controls (error bars indicate ± SD, n = 4–5 independent experiments, *= P < 0.05). (B) (Upper panel) Levels of periostin were analyzed through immunoblotting of fibroblast lysates using an anti-periostin antibody. (Middle panel) Levels of DDX41 were analyzed using an anti-DDX41 antibody. Arrowhead indicates a post-translationally modified form of DDX41. (Lower panel) immunoblotting of lysates with anti-vinculin confirmed equal loading of the samples. The positions of molecular weight markers in kilodaltons are shown. (C) The binding of p21 and POSTN mRNA to DDX41 protein was measured using a ribonucleoprotein immunoprecipitation assay. DDX41 was immunoprecipitated from total fibroblasts lysates with an anti-DDX41 antibody. Binding to normal rabbit IgG was used as a negative control and to calculate the relative binding of p21 and POSTN mRNA to DDX41 protein. The binding of p21 mRNA to DDX41 was used as a positive control (error bars indicate ± SD, n = 4–6 independent experiments, *= P < 0.05)