Figure A1. Activation of KDA11-01 and KDA11-02 TCR-Transduced Jurkat Cells. Flow cytometry analysis was used to assess the activation of TCR-transduced Jurkat reporter cells in response to specific antigens. (A) shows KDA11-01 TCR-transduced Jurkat reporter cells co-incubated with T2KO-A1101 cells without KD2 peptide loading, serving as a control to establish the gating strategy. (B) demonstrates the activation of KDA11-01 TCR-transduced Jurkat reporter cells following co-incubation with T2KO-A1101 cells loaded with 10⁻⁵ M KD2 peptide, indicated by the detection of reporter gene activation through flow cytometry. (C) shows KDA11-02 TCR-transduced Jurkat reporter cells co-incubated with T2KO-A1101 cells without KD2 peptide loading, confirming the gating strategy. (D) illustrates the activation of KDA11-02 TCR-transduced Jurkat reporter cells after co-incubation with T2KO-A1101 cells loaded with 10⁻⁵ M KD2 peptide, with reporter gene activation measured by flow cytometry.