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. 2024 Nov 19;14:28578. doi: 10.1038/s41598-024-79367-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — Excessive AP001007 alleviated LPS-triggered extra apoptosis in HK-2 cells. (A) Annexin V/PI staining results showing the apoptosis of HK-2 cells with the indicated treatments. (B) Quantification of the apoptosis results. (C,D) qPCR data depicting the expression of Bax (C) and Bcl-2 mRNA levels (D) in HK-2 cells with the indicated treatments. (E) Western blotting outcome illustrating the expression of Bcl-2, Bax, Cleaved-caspase 9, and Cleaved-caspase 3 in HK-2 cells with the indicated treatments. GAPDH was used as the internal control. (F) Quantification of the Western blotting outcome in (E). (G) Western blotting data indicating the levels of p65 and P-p65 in HK-2 cells with the indicated treatments. (H) Quantification of the Western blotting data in (G).