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. 2024 Oct 5;10(21):e39031. doi: 10.1016/j.heliyon.2024.e39031

Fig. 1.

Fig. 1

Que inhibits H/R-induced cardiomyocyte apoptosis and reduces cellular oxidative stress levels. (A) The effect of different concentrations of Que (0, 10, 20, 30, 40, 50, 60 μM) on the proliferation of normal cardiomyocytes was detected by CCK-8 assays. (B) The effects of different concentrations of Que (0, 10, 20, 30, 40, 50 μM) on cardiomyocyte proliferation in the H/R model were detected by CCK-8 assays (NC stands for normal H9C2). (C) TUNEL analysis of cardiomyocyte apoptosis (scale bar = 100 μm). (D) Western blot analysis of apoptosis-related proteins. (E) Detection of MDA levels by a kit. (F) Detection of SOD activity by a kit. (G) CAT activity was detected by a kit. (H) GSH-Px activity was detected by a kit. (I) Detection of LDH levels in cell supernatants. (J) Detection of CK levels in cell supernatants. ∗∗∗P < 0.001 vs. NC; △P < 0.05, △△P < 0.01, △△△P < 0.001 vs. H/R, n = 3. For the uncropped protein band plot, please see Fig. 1D in the Supplementary Material.