Fig. 4.

Effect of Que on myocardial mitochondrial function in MI/RI rats. (A) Western blot analysis of tissue Sirt3, SOD2 and AC-SOD2 protein expression. (B) Mitochondrial ROS levels were detected by flow cytometry. (C) ATP levels. (D) Cyto-c expression was detected by Western blotting. (E) Evans blue/TTC double staining to assess myocardial infarct size in rats. ∗∗∗P < 0.001 vs. sham; ^△P < 0.05, ^△△P < 0.01, ^△△△P < 0.001 vs. MI/RI. For the uncropped protein band plot, please see Fig. 4A–D in the Supplementary Material.