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. 2024 Oct 5;10(21):e39031. doi: 10.1016/j.heliyon.2024.e39031

Fig. 6.

Fig. 6

Que protects cardiac function through the Sirt3/SOD2 mitochondrial ROS pathway to inhibit cardiomyocyte apoptosis in MI/RI rats. (A) Western blot analysis of Sirt3, SOD2 and AC-SOD2 protein levels in tissue. (B) Mitochondrial ROS levels. (C) ATP levels in tissues. (D–G) Changes in oxidative stress indicators. (H) Detection of apoptosis by TUNEL assays (scale bar = 100 μm). Western blot analysis of apoptosis-related proteins (I) and Cyto-c (J) in tissues. (K–L) LDH and CK levels in rat serum. (M) H & E staining to assess myocardial histopathological changes (scale bar = 100 μm). (N) Assessment of the myocardial infarction area in rats by Evans/TTC double staining. ∗∗P < 0.01, ∗∗∗P < 0.001 vs. sham, △P < 0.05, △△P < 0.01, △△△P < 0.001 vs. MI/RI, #P < 0.01, ###P < 0.001 vs. MI/RI+3-TYP. For the uncropped protein band plot, please see Fig. 6A–I,J in the Supplementary Material.