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. 2024 Nov 20;13(11):659–672. doi: 10.1302/2046-3758.1311.BJR-2023-0361.R2

Fig. 3.

Fig. 3

Knockdown of PA28γ alleviates interleukin-1β (IL-1β)-induced dysregulation of anabolism and catabolism in chondrocytes. a) After transfection of chondrocytes with si-NC or si-PA28γ for 24 hrs, the knockdown efficiency of PA28γ was detected by quantitative real-time polymerase chain reaction (qRT-PCR) (****p < 0.0001). b) After transfection of chondrocytes with si-NC or si-PA28γ for 24 hrs, the knockdown efficiency of PA28γ was detected by western blotting (****p < 0.0001). c) mRNA expression of type II collagen (COL2) and SOX9 after transfection of chondrocytes with si-NC or si-PA28γ and treatment with IL-1β (**p < 0.01, ***p < 0.001). d) mRNA expression of matrix metalloproteinase-3 (MMP3) and MMP13 after transfection of chondrocytes with si-NC or si-PA28γ and treatment with IL-1β (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). e) Representative western blotting and quantitative data of COL2 and SOX9 after transfection of chondrocytes with si-NC or si-PA28γ and treatment with IL-1β (*p < 0.05, **p < 0.01, ***p < 0.001). f) Representative western blotting and quantitative data of MMP3 and MMP13 after transfection of chondrocytes with si-NC or si-PA28γ and treatment with IL-1β (*p < 0.05, ****p < 0.0001). g) Safranin O and toluidine blue staining image after transfection of chondrocytes with si-NC or si-PA28γ and treatment with IL-1β, measuring scale = 100 μm, magnification: ×200.