Fig. 5.

Knockdown of PA28γ alleviates interleukin-1β (IL-1β)-induced endoplasmic reticulum (ER) stress in chondrocytes. a) Representative western blotting and quantitative data of GRP78, CHOP, PERK, p-PERK, IRE1α, and p-IRE1α after transfection of chondrocytes with si-NC or si-PA28γ and treatment with IL-1β (***p < 0.001, ****p < 0.0001). b) CHOP representative immunofluorescence images of chondrocytes transfected with si-NC or si-PA28γ and treated with IL-1β, measuring scale = 100 μm. c) Representative western blotting and quantitative data of IKKβ, p-IKKαβ, P65, and p-P65 after transfection of chondrocytes with si-NC or si-PA28γ and treatment with IL-1β (*p < 0.05, **p < 0.01, ***p < 0.001). d) Representative western blotting and quantitative data of tumour necrosis factor receptor-associated factor 2 (TRAF2), JNK, p-JNK, ERK, p-ERK, P38, and p-P38 after transfection of chondrocytes with si-NC or si-PA28γ and treatment with IL-1β (*p < 0.05, **p < 0.01, ***p < 0.001). DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ns, not significant.