Fig. 1.

Increased GPR91 expression in the inflammatory periodontal tissues. (a) Gene transcription level of GPR91 in normal control (NC, n = 10) and gingival tissues with chronic periodontitis (CP, n = 10) as measured by real-time qPCR. (b) Protein expression of GPR91 in the gingival tissue from normal control (n = 2) or chronic periodontitis (n = 2) were evaluated by Western Blot. More details were shown in the Supplemental Information S1. Gingival tissue from normal control (n = 5) and chronic periodontitis (n = 5) were stained by immunofluorescence (c) and immunohistochemistry (d) stainning. In all cases, bars in graphs represent mean ± SEM. The images were collected from different gels with the same number of samples. NC:normal control,CP:chronic periodontitis. The data were analyzed by Student's t-test, and values are presented as the mean ± SD; *, p < 0.05; **, p < 0.01. Figures h and p represent the expression of GPR91 in the gingival tissue of the normal control group and chronic periodontitis group at low magnification, respectively. Figures h1/2 represent the expression of GPR91 in the epithelial and connective tissue of the normal control group at higher magnification. Figures p1-2 represent the expression of GPR91 in the epithelial tissue and connective tissue of the chronic periodontitis group at higher magnifcation.