Fig. 5.

GPR91-mediated inflammatory responses via the Erk/NFκb signaling pathway in P. gingivalis-treated BMDMs. (a) Protein expression was detected by Western Blot after P. gingivalis infection (MOI = 10, 50 or 250) at 1 h in BMDMs from WT mice. (b) BMDMs from WT mice were pretreated with 4C (GPR91 antagonist, 5 μM) for 2 h, then infected with P. gingivalis (MOI = 50) for 1 h, and the expression of BMDM protein levels was detected by Western Blot. (c) BMDMs from GPR91^−/− and WT mice were infected with P. gingivalis (MOI = 50) for 1 h, and the protein expression was detected by Western Blot. More details were shown in the Supplemental Information S1. BMDMs from GPR91^−/− and WT mice were infected with P. gingivalis (MOI = 50) for 12 h (d&e) The expression of p-Erk was detected by confocal imaging. (f&g) The expression of p-P65 was detected by confocal imaging at 12 h. Image is of a representative Western Blot form one independent experiment. Western Blot and immunofluorescence were performed three times independently. The scale bar represents 20 μm.