Fig. 4.

Effects of lipoxin A₄ on cardiac fibrosis in diabetic mice. A Representative image of the left ventricle with picrosirius red staining. Scale bar = 1 mm. B–D Quantification of the entire left ventricle, interstitial and perivascular collagen deposition in the indicated groups. E mRNA levels of fibrotic markers. F Representative LV immunoblot of fibronectin, collagen III and calnexin. G LV protein expression of fibronectin and collagen III. Data are presented as mean ± SEM. Two-way ANOVA followed by Fisher’s LSD post hoc test was used to compare the effects of phenotype and treatment. *P < 0.05, **P < 0.01. Ccn2, connective tissue growth factor; Mmp9, matrix metallopeptidase 9; Mmp2, metallopeptidase 2; Fn, fibronectin; Vegf, vascular endothelial growth factor