Figure 4.

The proliferation, migration and antioxidant effect of GELNs on PDLFs. (A) Cell scratch test result of GELNs intervened PDLFs after 12 and 48h, scale bar: 1 mm. (B)The effect of GELNs on PDLFs invasion after 24h, scale bar: 100 µm. (C) Detection of intracellular ROS levels by DCFH-DA, scale bar: 200 µm. (D) EdU effects on proliferation of PDLFs, scale bar: 50 µm. (E) Quantitation of the migration results. (F) The number of invading cells are presented as percent of invading cells. (G) Quantitative measurements the relative fluorescence intensity. (H) Quantitative measurements the proliferation of EdU-labeled PDLFs. *, **,*** and **** indicate difference for p<0.05, p<0.01, p<0.001 and p<0.0001 in comparison to Ctrl, respectively. ^{#, ##,###}and ^#### indicate difference for p<0.05, p<0.01, p<0.001 and p<0.0001 in comparison to LPS, respectively. n=3.

Abbreviations: DCFH-DA, 2’,7’-Dichlorofluorescin diacetate; PDLFs, periodontal ligament fibroblasts; ROS, reactive oxygen species.