Figure 2.

Concentration-response curves to U-46619 (A) and Phenylephrine (Phe, B) in femoral artery rings from Wistar rats in the absence (−muscle) or presence (+muscle) of the skeletal muscle. Concentration-response curves to Phenylephrine (Phe) was performed in femoral artery rings from Wistar rats in the absence (−muscle) or presence (+muscle) of the skeletal muscle with and without endothelium functional (C). Concentration-response curves to Serotonin (5-HT) was performed in rings without skeletal muscle (−muscle), with attached muscle (artery connected to the muscle), and detached muscle (poisoned near the artery in the chamber). Mesenteric superior (E), Basilar (F), and Carotid (G) were mounted in the presence of the skeletal muscle surrounding the femoral artery. The number of animals used in each experiment (n) is expressed in parentheses. The results are expressed as the mean ± SEM. Statistic: two-way ANOVA *P < 0.05; **P < 0.01. Please note that in some cases, the controls [presence or absence of muscle (−/+Muscle)] are consistent across all graphs, as the first myograph chamber was used as the control, while the other chambers were exposed to different inhibitors. To enhance clarity for the reader, we have presented these results in separate graphs.