Figure 4.

Concentration-response curves to Phenylephrine (Phe) performed in femoral artery rings from male and female Wistar rats in the absence (−muscle) or presence (+muscle) of the skeletal muscle, with the lactate dehydrogenase inhibitor [GSK2837808A, (GSK); A and D], and the monocarboxylic acid transport inhibitor [2-Cyano-3-(4-hydroxyphenyl)-2-propenoic acid (αCCA); B and E] and the neuronal nitric oxide synthase inhibitor [N-propyl-l-arginine (L-NPA); C and F]. Representative figure with the signaling pathways was blocked in the vascular reactivity experiment (G). Typical recording of a concentration-response curve to lactate (10 µm-25 mm) in the femoral artery (−muscle) rings pre-contracted with KCl (90 mm) (H). The number of animals used in each experiment (n) is expressed in parentheses. The results are expressed as the mean ± SEM. Statistic: 2-way ANOVA or Student’s t-test as appropriate, *P < 0.05; **P < 0.01. Please note that in some cases, the controls [presence or absence of muscle (−/+Muscle)] are consistent across all graphs, as the first myograph chamber was used as the control, while the other chambers were exposed to different inhibitors. To enhance clarity for the reader, we have presented these results in separate graphs.