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. 2024 Sep 17;5(6):zqae042. doi: 10.1093/function/zqae042

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© The Author(s) 2024. Published by Oxford University Press on behalf of American Physiological Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Immunofluorescence staining for MyHC (Myosin heavy chain, A) and NOS1 (neuronal nitric oxide synthase, B) in cells from skeletal muscles surrounding the carotid (sternohyoid) and femoral (adductors) arteries from Wistar rats. Representative images shown (MyHC, green; NOS1, pink; DAPI, blue), original magnification 20×. The Representative figure shows the protocol performed with skeletal muscle cells, which were isolated, cultured, and treated with lactate (5 mm, 1 h) or vehicle (C). Graphical representation of the expression of total NOS1, Ser1417, Ser847 and GAPDH as loading control (D). Relative amounts of total NOS1 (E), Ser1417 (F), and Ser847 (G) proteins were shown by densitometry. Each lane was loaded with 50 μg of total protein. The number of animals used in each experiment (n) is expressed in dots. The results are expressed as the mean ± SEM. Statistic: Student’s t-test, ^****P < 0.0001. Please note that the original membrane referenced in Figure 5 are in the Suppl. Figure S1.