Fig. 4.

PN inhibited the proliferation of HNSCC via the JNK pathway. A-B Western blotting detected the key protein levels of the JNK pathway in PN + L-treated Cal27 and Tu686 cells, along with the statistical analysis. C-D Protein changes of p-JNK, c-Fos and c-Jun in Cal27 and Tu686 cells treated or untreated with PN + L and SP600125 were detected by Western blotting. Statistical analysis is presented. E CCK-8 assay was used to detect the cell viability of Cal27 and Tu686 cells treated with PN + L for 48 h with or without SP600125. F-G Colony formation assay of PN + L-treated HNSCC cells with or without SP600125 treatment. Quantitative analysis of colony numbers. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data are shown as the mean ± SD from three independent experiments