Fig. 5.

PN induced apoptosis and inhibited EMT ability via the JNK pathway. A-B Cal27 and Tu686 cells treated or untreated with PN + L and SP600125, and the apoptosis rate was assessed by flow cytometry. C-D Wound-healing assay was used to detect the migration of PN + L-treated HNSCC cells with or without SP600125. Scale bar: 50 μm. E-G Cal27 and Tu686 cells treated or untreated with PN + L and SP600125, and the protein levels of Bax, Bcl-2, E-cadherin, N-cadherin and Vimentin were assessed using Western blotting. Statistical analysis is presented. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data are shown as the mean ± SD from three independent experiments