Fig. 6.

PN and PND exerted antitumour effect on HNSCC in vivo. A The procedure for the xenograft tumour model in nude mice. B The body weight of nude mice was measured every 2 days. C Tumour volume was measured every 2 days. D The tumour inhibition rate was calculated after death of nude mice. E Morphological images of xenograft tumours. F Tumour weight at the end of the experiment. G HE staining of transplanted tumours, heart, liver, lung, and kidney in nude mice. Scale bar: 50 μm. H-I IF staining of Bax, Bcl-2, E-cadherin, N-cadherin and Vimentin, p-ASK1, p-JNK, c-Fos and c-Jun, along with quantitative analysis of staining intensity. Scale bar: 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data are shown as the mean ± SD from three independent experiments