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. 2024 Nov 19;25:88. doi: 10.1186/s40360-024-00810-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Reduction in Beas-2B cell viability through RES-mediated inhibition of heat stress and oxidative stress induction. (A) Phase contrast microscopic observation of the effect of different temperature water bath treatments on cell states. (B) MTT assay results regarding the effect of RES on cell viability at 37 °C, 38 °C, 40 °C, and 42 °C; (C) RES molecular 3D structure and molecular formula; (D) MTT assay results regarding the effects of different doses of RES on the viability of Beas-2B cells. (E) MTT assay results regarding the effects of different doses of RES on the viability of Beas-2B cells under heat stress at 42 °C. (F-H) Biochemical assay for the determination of MDA, GSH, and LDH in cells. (I) Flow cytometric detection of fluorescence intensity of the ROS fluorescent probe DCFH-Da. (J) FlowJo V10. quantitative statistics of flow cytometry assay results; **P < 0.01, *P < 0.05, compared with the 37 °C group or control group; ##P < 0.01, #P < 0.05, compared with the 42 °C group, the differences were statistically significant