Fig. 3.

Nrf2-IN-1 significantly reverses the resistance of RES to ferroptosis in Beas-2B cells under heat stress. (A) Prediction of Nrf2 protein active pocket sites using Deepsite. (B) Visual simulation docking of potential binding relationship between RES and Nrf2 active pocket sites using Pymol. (C) MTT assay for cell viability. (D) LDH levels, (E) intracellular Fe²⁺ content. (F) Results of western blotting. (G–L) Quantitative statistics of protein band optical density values determined using western blotting. **P < 0.01, *P < 0.05, compared with the 37 °C group; ##P < 0.01, #P < 0.05, the two groups were compared