Fig. 1.

The CDPK conserved domain in land plants. a. EF-hand and autoinhibitory structures in different CDPK protein classes. The sequence logos were generated by the WebLogo online tool (https://weblogo.berkeley.edu/logo.cgi) based on the protein class alignments constructed using Mega11. The overall height of each stack letter indicates the sequence conservation at that position (measured in bits), whereas the height of the symbols within each stack reflects the relative frequency of the corresponding amino acid at that position. The red-marked region shows the conserved domains. The far-left tree was not drawn to scale and does not represent any phylogenetic distributions; b. Evolutionary relationships of the CDPK gene family in 52 land plants. An unrooted NJT tree was constructed with full protein sequences in MEGA X software at a bootstrap value of 1000, and the other parameters were held constant. Phylogenic classes were designated based on protein clustering and previous publications. Different color schemes show different CDPK protein classes. Different groups and subgroups are labeled in the figure