Figure 2.

PDGFβ signaling is blocked by imatinib. TED OFs were pretreated with imatinib (5 µM) for 1 hour followed by stimulation with PDGFβ (25 ng/mL) for 48 hours. (A) Cells were lysed and analyzed by Western blot for phospho-AKT and total AKT. Phospho-AKT was normalized to total AKT. (B) Quantification of Western blots for phospho-AKT from three TED OF strains. (C) TYMS, AHR, and CYP1B1 were analyzed by Western blot with levels normalized to β-Tubulin. (D) Quantification of Western blots for TYMS, AHR, and CYP1B1. Uncropped blots are presented in the Supplementary Data File. Vehicle (DMSO) treated cells served as control The experiment was repeated in three different TED OF strains with representative results shown. One-way ANOVA with Dunnett's multiple comparisons was used to analyze the data. * P ≤ 0.05, ** P = 0.005, *** P < 0.0008, and PDGF versus treatments: # P ≤ 0.05, ## P ≤ 0.005.