Fig 2. Construction of uORF-connected transcripts from a uORF and a downstream protein-coding transcript.

The original protein-coding sequence is shown in green rectangles. For uORF-connected transcript #1, a splice junction (red bars) found in the GTEx collection of RNA-seq data is used to link the uORF to the second exon of the downstream transcript. For uORF-connected transcript #2, a splice donor (SD) site predicted by Splam (blue bars) is paired with an annotated splice acceptor (SA) site in a MANE transcript. The novel protein sequences are shown in red rectangles.