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. 2024 Nov 20;13:RP94347. doi: 10.7554/eLife.94347

Figure 2. IGF2BP2 positively regulates Zika virus (ZIKV) replication in multiple cell lines.

Figure 2.

Liver Huh7.5 (A–C), astrocytic NHA-hTERT (D), and placental JEG-3 (E) cells were transduced with non-target shRNA (shNT) or shIGF2BP2 lentiviruses at an MOI of 10. Two days post-transduction, cells were infected with ZIKV H/PF/2013 at an MOI between 0.01 and 1 depending on the cell line. Two days post-infection, supernatant and cells were collected. IGF2BP2 expression at the protein level (A, D, E; all cell lines) and mRNA level (B; Huh7.5 cells) were evaluated by western blotting (WB) and RT-qPCR, respectively. Cell supernatants were used for plaque assays (C–E). For NHA-hTERT and JEG-3, the supernatant and cells are collected for titration and WB, respectively (E–F). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were performed to assess the cell viability in transduced NHA-hTERT and JEG-3 cells (D–E). Means ± SEM are shown based on five (D), three (C), and four (D–E) independent experiments. ****: p<0.0001; ***: p<0.001; **: p<0.01 (unpaired t-test).

Figure 2—source data 1. Data points to generate the bar graphs of Figure 2B–E.
Figure 2—source data 2. PDF file containing original western blots for Figure 2A and D–E, indicating the relevant bands and conditions.
Figure 2—source data 3. Original files for western blot analysis displayed in Figure 2A and D–E.