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. 2024 Nov 20;13:RP96695. doi: 10.7554/eLife.96695

Figure 1. Cell length distribution of M.smegmatis cells treated with different drugs.

Horizontal lines represent the mean of the plotted data points (n=84–212). The inset shows the fold changes in cell length compared to the untreated control on a log2 axis, highlighting the phenotypic effect of each treatment. * indicates data significantly different from the control at p=0.0001. Numerical values and additional statistical parameters are provided in Figure 1—source data 1.

Figure 1—source data 1. Cell dimensions of M. smegmatis treated with different drugs.

Figure 1.

Figure 1—figure supplement 1. Treatment optimization in liquid culture.

Figure 1—figure supplement 1.

Effects of treatment on Mycobacterium smegmatis mc2-155 wild-type liquid cultures during the exponential growth phase. Relative growth inhibition is expressed as the % OD600 ratio between treated and control liquid cultures for isoniazid (INH), ethambutol (EMB), ethambutol (RIF), and pyrazinamide (PZA) (latter grown in pH 5.5 acidic media). For ciprofloxacin (CIP) and mitomycinC (MMC), it is represented as the % ratio of colony-forming units (CFUs) following an 8 hr treatment.
Figure 1—figure supplement 2. Treatment optimization on agar plates.

Figure 1—figure supplement 2.

Relative viability is calculated based on the colony-forming unit (CFU) counting of exponentially growing wild-type M. smegmatis cultures on control and selective plates containing the corresponding antibiotic at different concentrations. Incubation: 60 hr at 37°C.