Figure 5. Phenotypic ciprofloxacin (CIP) tolerance assay.

(A) Scheme of the fluctuation test used in the study. (B) Development of phenotypic resistance to a selecting CIP concentration following preincubation with a sublethal CIP concentration for various time periods. Data bars represent the averages of three biological replicates each carried out in three technical replicates; error bars represent SE.

Figure 5—figure supplement 1. Ciprofloxacin (CIP) tolerance of M. smegmatis preincubated for 96 hr on CIP-containing plates sent for Whole Genome Sequencing (WGS).

Wild-type (WT) M. smegmatis colony was cultivated in LEMCO broth to reach OD = 0.4–0.5, then striked onto plates containing 0.5 µg/ml CIP; 0.3 µg/ml CIP or onto non-selecting plates and grown for 4 days at 37 °C. Data bars represent the averages of three biological replicates each carried out in three technical replicates; error bars represent SE.