Figure 3.

CB-pDCs resemble primary pDCs at the transcriptional level. (A) UMAP plot showing the immune cell subsets in CB-DCs and primary pan-DCs. (B) UMAP plot showing pDCs resolved from enriched pan-DC and CB-DC samples. (C) UMAP plot showing the gene expression levels of different pDC markers. NA stands for not applicable and refers to cells not annotated as pDCs due to the lack of pDC marker expression. (D) Mean expression levels of selected pDC signature genes in the different immune cell subsets. Dot size indicates fraction of positive cells, color indicates mean expression levels. (E) GSEA analysis comparing CB-DCs of the different donors with the DC signatures published by Villani et al. Results of 3 independent untreated, unprimed CB donors and 3 independent pan-DC donors are depicted. See also Supplementary Figure S2 .