Figure 5.

TLR stimulation activates similar genes and pathways in CB and primary pDCs. (A) Cluster dendrogram depicting a hierarchical clustering analysis of the different donors comparing primary pDCs to CB-pDCs with or without priming upon TLR stimulation or left untreated. (B, C) Volcano plots showing differentially expressed genes (right: up-regulated, left: down-regulated) between untreated and upon (B) TLR9 or (C) TLR7 activation of various pDC types as indicated by labeling. Colors display significance (orange) and significance and high log fold change (red), n.s.: not significant (gray). (D) Dot plot depicting the fraction of positive cells (dot size) and their mean expression levels (dot color) of different donors and various treatments as indicated. Genes were selected based on function or activation in pDCs. (E) BubbleMap showing the most relevant pathways enriched in TLR9- and TLR7-activated CB-pDCs and primary pDCs. Color code as explained in the figure, increasing intensity represents stronger enrichment in TLR-treated groups (red) or in untreated controls (blue) with bubble sizes corresponding to the absolute values for the normalized enrichment score. Results of 3 independent CB donors and 3 independent pan-DC donors are shown, with unprimed and primed CB cells and primary pan-DCs with or without activation with TLR9 or TLR7 agonists for 4 hrs. See also Supplementary Figure S4 and Supplementary Table S3 .