Figure 6.

CB-pDCs can induce inflammation in co-culture with cells from CRC tumor digests. (A) Graphical representation of the experimental set-up of the CB-pDC/CRC co-culture. (B) Quantification of activation markers on CB-pDCs upon co-culture with CRC digests using flow cytometry. (C) Quantification of IFN-ɑ release in supernatant of CB-pDCs either from co-culture with CRC digests or cultured alone showing all indicated treatment groups. (D, E) Quantification of (D) cytokine and chemokine release and (E) release of cytotoxic mediators in supernatant of CB-pDCs either from co-culture with CRC digests or cultured alone of all indicated treatment groups. Colors displaying the minimum (0%) to maximum (100%) mean cytokine concentration per column. Results are shown as mean ± SEM of 2 independent experiments, with one independent CB donor and 4 independent CRC donors. *p>0.1, ns: not significant, one-way ANOVA with Tukey’s post-hoc test. See also Supplementary Figure S5 and Supplementary Table S1 .