Table 2.

Overview of samples included in the scRNA sequencing experiment.

Sample name	Number of replicates (different donors)	Description
Pan-DCs untreated	3	Pan-DCs enriched from PBMCs, left untreated.
Pan-DCs TLR9 treated	3	Pan-DCs enriched from PBMCs, treated with a TLR9 agonist for 4 hrs.
Pan-DCs TLR7 treated	3	Pan-DCs enriched from PBMCs, treated with a TLR7 agonist for 4 hrs.
CB-DCs unprimed untreated	3	Differentiated CB-DCs were left unprimed for 72 hrs and next, left untreated.
CB-DCs unprimed TLR9 treated	3	Differentiated CB-DCs were left unprimed for 72 hrs and next, treated with a TLR9 agonist for 4 hrs.
CB-DCs unprimed TLR7 treated	3	Differentiated CB-DCs were left unprimed for 72 hrs and next, treated with a TLR7 agonist for 4 hrs.
CB-DCs unprimed untreated	3	Differentiated CB-DCs were primed for 72 hrs and next, left untreated.
CB-DCs unprimed TLR9 treated	3	Differentiated CB-DCs were primed for 72 hrs and next, treated with a TLR9 agonist for 4 hrs.
CB-DCs unprimed TLR7 treated	3	Differentiated CB-DCs were primed for 72 hrs and next, treated with a TLR7 agonist for 4 hrs.