Figure 3.

Subcellular localization of known and putative iron transport proteins. Representative erythrocytes infected with P. falciparum 3D7 parasites endogenously expressing GFP-tagged PfMRS3 (A), PfVIT (B), PfZIPCO (C) or PfE140 (D) were additionally stained with the fluorescent dyes Hoechst-33342, MitoTracker Red, ER Tracker Red and/or LysoTracker Deep Red. Co-transfection with a construct that encodes the 60 N-terminal amino acids of acyl carrier protein (PfACP) tagged with mCherry (Birnbaum et al., 2020) resulted in red fluorescence of the apicoplast. Live-cell images were taken under physiological conditions at 37°C using an SP8 confocal laser-scanning microscope (Leica). DIC, differential interference contrast. Scale bar, 2 µm.