Fig. 2. ADAM9 bacteria are efficiently cleaved by rADAM9, rMMP9, and PDAC lysates.
The influence of thrombin and rADAM9 (A), rMMP9 alone or in the presence of the MMP inhibitor E3304 (B), and PANC-1 lysate (C) Incubation of ADAM9 bacteria on the percentage of SAPE-positive bacteria. D Fluorescence levels of SAPE-labeled ADAM9 bacteria upon PANC-1 and THP-1 lysate incubation under static conditions as measured by Biotek over time. Data are shown as the mean ± SD of one representative experiment with n = 2, except for (A) and (D), which represent two experiments with n = 2. Two-way ANOVA was used to compare % of events in Q1. Levels of significance: ns not significant, *** p < 0.001, **** p < 0.0001.