Fig. 4.

5-FU combined with simvastatin significantly inhibited the organoid growth. A Graphical representation of the gene identification and organoid construction of KPC mouse model. B-C The synergy of 5-FU and simvastatin in the KPC organoid model was assessed, and the optimal concentration and synergy score were determined. D Representative images of the KPC organoids’ response to 5-FU and simvastatin (5-FU: 100 nM; simvastatin: 100 nM). E–H Relative growth rate, area, and diameter of KPC organoids’ response to 5-FU and simvastatin (n = 6). Results are presented as mean ± standard deviation. Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001