Table 4.
Accuracy and precision for the quality controls for all included substances. From a single run on eight replicates at each quality control level (inter-day assay) and from eight different days (intra-day assay)
| Intra-day assay (n =8) | Inter-day assay (n =8) | ||||||
|---|---|---|---|---|---|---|---|
| Substance | QC I | QC II | QC III | QC I | QC II | QC III | |
| 0.1 nmol/L | 0.5 nmol/L | 3.75 nmol/L | 0.1 nmol/L | 0.5 nmol/L | 3.75 nmol/L | ||
| dTG |
Mean SD Imprecision (CV%) Accuracy (%) |
0.10 0.005 4.5 101.1 |
0.05 0.016 3.2 99.2 |
3.6 0.1 4.1 96.0 |
0.11 0.01 9.2 110.0 |
0.48 0.04 9.2 96.0 |
3.8 0.36 9.5 101.3 |
| QC I | QC II | QC III | QC I | QC II | QC III | ||
| 0.25 µmol/L | 1.3µmol/L | 9.4 µmol/L | 0.25 µmol/L | 1.3 µmol/L | 9.4 µmol/L | ||
| dC |
Mean SD Imprecision (CV%) Accuracy (%) |
0.27 0.01 5.1 108.0 |
1.37 0.04 3.0 105.4 |
9.9 0.48 4.9 105.3 |
0.25 0.03 10.9 100.0 |
1.28 0.11 8.4 98.5 |
9.9 0.9 9.1 105.3 |
Abbreviations: dTG, 2′-deoxythioguanosine; dC, 2′-deoxycytidine; QC, quality control; CV, coefficient of variation. Mean and SD in nmol/L for dTG and µmol/L for dC